S.BIOMEDICS

: Functionally Enhanced Cell Spheroid
FECS^TM is a technology that forms cell spheroids with enhanced functions as the unique technology name of S.Biomedics.

Why the 3D Cell Spheroid is Necessary?: In general, the 3D cell spheroids (3D cell cluster) are superior to 2D cultured cells.

Strength

Maintains the cell’s intrinsic functions
It allows maintaining the cell’s intrinsic functions as it is cultured through a similar formation as the form of the cells (micro-tissues) making up tissues in the body.
Cell engraftment and survival rate
The engraftment rate after administration increases due to the highly organized extracellular matrix and the survival rate of the cells increases accordingly, which results in an excellent therapeutic effect.
Generates various effector molecules from the cells
The amount of effector molecules generated by cells (cytokines, growth factors, etc.) increases due to the high density of the cells and abundance of the extracellular matrix that mediates intercellular signaling.
Formation efficiency of the 3D cell spheroid
Most of the seeded cells should be formed into one spheroid and the number of the cells within the spheroid should also be consistent.
Morphological uniformity and reproducibility
The size and shape should be uniform and maintained at each manufacturing.
Homogeneity of cellular properties within the spheroid
A homogeneous micro-environment of the cell should be created with no differences in nutrient supply between cells making up the inside and outside of the spheroid.

OUTLINE

Stage 1: Producing and coating bioactive proteins

Recombinant bioactive proteins are made up of hydrophilic and hydrophobic ends. The hydrophobic end binds to the culture dish while the hydrophilic end binds to the cell.
Stage 2: Cell seeding and self-organization

When the cells are seeded into a culture dish coated with bioactive proteins, the binding force between the bioactive protein and cell becomes weaker than that between the cells. Accordingly, the seeded cells are self-organized and form a 3D cell spheroid.

DATA

Self-organization

FECS^TM technology maximizes cell-cell interaction and thereby induces self- organization into a cell spheroid. The time taken to form a spheroid is relatively short - within 24 hours after cell seeding. It is characterized by the formation of an intact sphere shape (spheroid). As self- organization is completed without artificial force to the cell, an abundant extracellular matrix is expressed in the cell spheroid that has been formed.

Size Adjustment

The size of the spheroid can be adjusted in proportion to the size of the culture dish and the number of cells. Therefore, the cell spheroid of proper sizes for different diseases can be used.

Number of culture dish wells (culture dish well size)

12(3.8cm²) 24(1.9cm²) 48(0.95cm²) 96(0.32cm²)
number of cells per well

6,000 Cells 7,000 Cells 8,000 Cells 9,000 Cells 10,000 Cells 11,000 Cells 13,000 Cells 15,000 Cells

Consistent Reproducibility

The formation rate, shape, and size of the generated cell spheroids are constant, and it can be cultured in a three-dimensional structure that is the most similar to the environment in the human body. Therefore, excellent engraftment rate and survival rate inside the tissue are expected after injecting the cell therapy products.

Formation rate Most of the seeded cells (over 99.9%) form a spheroid.

Size Both the width and height of the cell spheroid formed are consistent at about 450 μm, and the sizes of the spheroids are uniform.

Shape All of the cell spheroids formed show an eccentricity close to zero and are formed in a constant sphere.

Abundant Extracellular Matrix

Scanning electron microscope (SEM) As self-organization is possible without applying artificial force during the formation of 3D structure, FECS™ technology induces abundant expression of the extracellular matrix that makes up the spheroid which is highly organized.

Immunofluorescence staining The extracellular matrix is distributed across the entire spheroid. A highly dense network of collagen, laminin, etc. is expected to make a great contribution to healing wounds and regenerating tissues.

DAPI Col-1 LN Merged

Secretion of Tissue Regeneration Factors

When the secretion of tissue regeneration factors in each culture technique is compared, the production rate of cytokines and growth factors is superior in FECS^TM technology than that of 2D culture and other 3D technology.

Versatility: FECS^TM technology can be applied to most cells, which increases the possibility of developing new technologies.
Platform technology through the collaboration in the way of “Plug-In / Plug-Out”
Specific cells owned by other companies and laboratories can be integrated with the FECS™ technology for the new medicine R&D.