In general, the 3D cell spheroids (3D cell cluster) are superior to 2D cultured cells.
Recombinant bioactive proteins are made up of hydrophilic and hydrophobic ends. The hydrophobic end binds to the culture dish while the hydrophilic end binds to the cell.
When the cells are seeded into a culture dish coated with bioactive proteins, the binding force between the bioactive protein and cell becomes weaker than that between the cells. Accordingly, the seeded cells are self-organized and form a 3D cell spheroid.
FECSTM technology maximizes cell-cell interaction and thereby induces self- organization into a cell spheroid. The time taken to form a spheroid is relatively short - within 24 hours after cell seeding. It is characterized by the formation of an intact sphere shape (spheroid). As self- organization is completed without artificial force to the cell, an abundant extracellular matrix is expressed in the cell spheroid that has been formed.
The size of the spheroid can be adjusted in proportion to the size of the culture dish and the number of cells. Therefore, the cell spheroid of proper sizes for different diseases can be used.
The formation rate, shape, and size of the generated cell spheroids are constant, and it can be cultured in a three-dimensional structure that is the most similar to the environment in the human body. Therefore, excellent engraftment rate and survival rate inside the tissue are expected after injecting the cell therapy products.
Most of the seeded cells (over 99.9%) form a spheroid.
Size Both the width and height of the cell spheroid formed are consistent at about 450 μm, and the sizes of the spheroids are uniform.
Shape All of the cell spheroids formed show an eccentricity close to zero and are formed in a constant sphere.
Scanning electron microscope (SEM) As self-organization is possible without applying artificial force during the formation of 3D structure, FECS™ technology induces abundant expression of the extracellular matrix that makes up the spheroid which is highly organized.
Immunofluorescence staining The extracellular matrix is distributed across the entire spheroid. A highly dense network of collagen, laminin, etc. is expected to make a great contribution to healing wounds and regenerating tissues.
When the secretion of tissue regeneration factors in each culture technique is compared, the production rate of cytokines and growth factors is superior in FECSTM technology than that of 2D culture and other 3D technology.
Specific cells owned by other companies and laboratories can be integrated with the FECS™ technology for the new medicine R&D.