R & D

3-D Microtissue

Cause

The traditional cell culturing method physically or chemically separates the cells of the human tissue and cultures them on a two-dimensional surface or medium. In this process, the enzymes and chemicals used during the culturing process damage the cell surface and the cultured cells lose the characteristics of typical cells in the human body.

Process

Our technology is to coat the surface of the two-dimensional culture with a special material so that the cells in time will cultivate into three-dimensional microstructures.

Expectation

When applied to skin cells, the amount of extracellular matrix (collagen, etc) increases compared to cells cultured by conventional methods, and the proliferation of cells can be performed without causing contraction of surrounding tissues when injected into the skin. When applied to cancer cells, they can be cultured as cells having the characteristics of cancer tissues in vitro, and can be used for screening of anticancer drugs and such. In the future, we will develop therapeutic and cosmetic test models for skin and adult stem cells, and develop drug screening models applicable to cancer cells.

Formation and characteristics of 3D microstructure

Result

In the cultivation of dermal fibroblasts, 3D microstructures are formed with the passage of time. The formed microstructures possess more abundance of collagen, fibronectin, and elastin, in extracellular matrix when compared with the 2D culture method. Vascular endothelial growth factor (VEGF) production also increased.

Control of size of 3D microstructure

The microstructure size can be controlled, and thus can be applied to many different fields.